Professor Susan Sharfstein and alumna publish collaborative CHO cell productivity research in Antibody Therapeutics
SUNY Poly professor of nanobioscience Dr. Susan Sharfstein, with alumna Sarah Nicoletti and researchers from the National Institute for Innovation in Manufacturing Biopharmaceuticals; the School of Biomedical Sciences, Ulster University; and the Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland; published Chinese Hamster Ovary (CHO) cell-based research in Antibody Therapeutics.
The team indicated in the publication, "We investigated the role of DNA CpG methylation in various positions along the CMV promoter in high-producing CHO cells. Our results indicate that despite vector elements and repeated rounds of MTX screening, higher producers show a counterintuitive increased methylation of the CMV promoter region, particularly near transcription factor binding sites."
The work aims to assist with a bottleneck in the biopharmaceutical manufacturing process, the identification of “good cell lines” for bioproduction, thus reducing the need for brute force screening by increasing our biological understanding of the factors that affect productivity.